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	Provedor de dados ArchiMer (1) Autor Bechemin, Christian (1) Collinot, Cecile (1) Delmas, Daniel (1) Legrand, Catherine (1) Palavra-chave Activité exoprotéolytique (1) Analyse en flux continu (1) Bacterioplankton (1) Bactérioplancton (1) Coastal marine pond (1) Dissolved protein (1) Exoproteolytic activity (1) Flow injection analysis (1) Marais maritime (1) Protéine dissoute (1) Mais... Tipo do documento Text (1) Ano 1994 (1) País France (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Exoproteolytic activity determined by flow injection analysis: its potential importance for bacterial growth in coastal marine ponds ArchiMer Delmas, Daniel; Legrand, Catherine; Bechemin, Christian; Collinot, Cecile. The measurement of the fluorescent 4-methyl-7-coumarinylamine released from the hydrolysis of a non-fluorescent peptide model substrate by exoproteolytic enzymes has been adapted to flow injection analysis (FIA). FIA allows samples to be processed very quickly (less than 2 min. for triplicate determinations) with good sensitivity (< 0.1 muM) and reproducibility (relative standard deviation < 3 % at the 0.2 muM level). In a coastal marine pond, exoproteolytic activity was closely related to bacterioplankton biomass. The high activity measured in pond water (maximum velocity: V(M) almost-equal-to 1.46 to 2.54 muM.h-1) emphasizes the importance of dissolved protein hydrolysis for bacterial growth and for dissolved organic nitrogen cycling. The mean... Tipo: Text Palavras-chave: Activité exoprotéolytique; Protéine dissoute; Analyse en flux continu; Bactérioplancton; Marais maritime; Flow injection analysis; Exoproteolytic activity; Dissolved protein; Bacterioplankton; Coastal marine pond. Ano: 1994 URL: http://archimer.ifremer.fr/doc/00196/30756/29163.pdf Registros recuperados: 1 Primeira ... 1 ... Última

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